HEK-293 Cell Culture Media (Viral Vectors Production) ​​

HEK-293 cells (Human Embryonic Kidney cells) are widely utilized for recombinant protein production and viral vector production (e.g., Adeno-Associated Virus AAV, Adenovirus AdV, Lentivirus LV). To meet their culture requirements, the CD 293 series products (including CD 293 06 medium) are manufactured under GMP conditions. These are chemically defined, animal component-free media that effectively support high-density growth and efficient transfection/expression in various HEK-293 cell lines.

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Product Catalog

Product Catalog No. Form Package Size Description User Guide Inquiry and Trial
CD 293 01 11203-1238、11203-22052 Dry Powder、Liquid 500 mL、1000 mL、2 L、10 L、50 L、100 L、Customized Basal Medium /
CD 293 06 11207-1652、11207-24018 Dry Powder、Liquid 500 mL、1000 mL、2 L、10 L、50 L、100 L、Customized Basal Medium /
CD 293 FA 99151-1541、99151-22055 Dry Powder、Liquid 500 mL、1000 mL、2 L、10 L、50 L、100 L、Customized Feed, used with CD 293 FB mainly for stable transfection expression of HEK-293 cells /
CD 293 FC 99151-1327、99151-23016 Dry Powder、Liquid 500 mL、1000 mL、2 L、10 L、50 L、100 L、Customized Feed, used with CD 293 FB mainly for stable transfection expression of HEK-293 cells /
CD 293 FB 99035-1242、9035-23004 Dry Powder、Liquid 500 mL、1000 mL、2 L、10 L、50 L、100 L、Customized Feed, used with CD 293 FA or FC, the dosage is 1/10 of CD 293 FA or FC /
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Case Studies

HEK-293 Cell Growth​

CD 293 01 medium enables robust growth of HEK-293 cells, with a doubling time of approximately 21-24 hours and cell viability exceeding 90%. In batch culture, the maximum cell density can reach 14.0 × 106cells/mL.

Figure 1.
a) Passage culture of HEK-293 cells using different media, with a seeding density of 0.5 × 106 cells/mL and passage once every 3 days;
b) Batch culture of HEK-293 cells using different media, with a seeding density of 0.5 × 106 cells/mL.

Transfection Efficiency of HEK-293 Cells

Figure 3.
a) FSC-A (cell size)/SSC-A (cell granularity) scatter plot, gating to select P1 cell population;
b) Distribution of fluorescently labeled positive cells in P1 population (positive rate 91.18%).

Viral Vector Production of HEK-293 Cells

Case 1

Table 1. CD 293 06 demonstrated superior performance in viral vector titer (3.00E11 vg/mL) and full capsid percentage (10) compared to competitors, while all exhibited 95% purity.

Case 2

Figure 4. Preparation of viral vectors by HEK-293 cells using different media. a) AAV; b) LV. CD 293 06 medium can significantly increase the yield of viral vectors. The yield of AAV can reach 10¹¹ vg/mL, and that of LV can reach 10⁷ TU/mL, and the yield of CD 293 06 medium is significantly higher than that of competitors.