Stem Cell Culture Media

This medium promotes rapid MSC proliferation while maintaining stable morphology.

Figure 1. Morphology of umbilical cord–derived MSCs (UC-MSCs, left) and placenta-derived MSCs (PL-MSCs, right) cultured in Stem Cell 1, recorded using inverted microscopy.

Stem SFM 01

When culturing adipose-derived stem cells, Stem SFM 01 supplemented with only 1.5% fetal bovine serum produced cell morphology (Figure 2) and density (Figure 3) comparable to alpha-MEM supplemented with high concentrations of serum substitute, significantly reducing serum dependence and improving cost-effectiveness.

Figure 2. Morphology of adipose-derived stem cells on microcarriers.

Figure 3. Comparison of viable cell densities of adipose-derived stem cells.

Table 1. Comparison of stem cell growth in Stem SFM 01 versus competitor media at passage 7. Stem SFM 01 showed superior viable cell density and fold expansion.

Stem Cell 1

MSCs expanded in Stem Cell 1 while retaining high purity.

Table 2. Flow cytometric detection of surface markers of MSCs cultured to passage 5 (P5) in Stem Cell 1.

Stem SFM 01

Figure 4. Positive marker expression exceeded 99% (CD73/CD90/CD105), while negative marker expression (CD34/CD45/HLA-DR) remained below 1%.

Stem Cell 1

Figure 5. Adipogenic and osteogenic induction experiments were performed using P5 UC-MSCs and PL-MSCs. Adipocytes were stained with neutral lipid dye (green), and osteoblasts were stained with alizarin red (red). MSCs at passage 5 retained multi-lineage differentiation potential.

Stem SFM 01

Table 3. The exosome yield of Stem SFM 01 medium is 2.24×10¹² particles/L, higher than that of an MNC medium (2-5×10¹¹ particles/L).

Figure 6. Comparison of exosome native solutions: exosomes produced in Stem SFM 01 exhibited superior productivity, along with uniform vesicle morphology and distinct structural features, indicating that Stem SFM 01 efficiently supports stem cells in secreting high-quality exosome