F81 Cell Culture Media

F81 LSM 801 is a low-serum medium formulated to support the suspension growth of F81 cells. During the cell expansion phase, serum supplementation is limited to 2%–3%. This medium is suitable for serial passaging, scale-up, and virus production using F81 cells.

Our Features

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Our Strengths

TSE/BSE declaration can be provided
Production meets cGMP standards
Consistently excellent batch-to-batch quality
Flexible packaging selection
Significant increase in product yield
Three major international manufacture sites ensure fast and stable supply
Customizable components of catalog culture media

Product Catalog

Product	Catalog No.	Form	Package Size	Description	User Guide
F81 LSM 801	11401-801	Dry Powder	2 L, 10 L, 50 L, 100 L, Customized	Low-serum cell culture medium	Download
ExpVirus A	99166-22039	Liquid	500 mL,1000 mL	Feed	/
ExpVirus B	99167-22040	Liquid	500 mL,1000 mL	Feed	/

Inquiry and Trial

Frequently Asked Questio

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Case Studies

Adherent cell suspension adaptation

F81 LSM 801 medium enables stable serial passaging of F81 cells, with a doubling time of approximately 35 hours. In batch culture, the medium maintained high-density F81 cell growth, with cell viability consistently above 98% during the first 9 days.

a) b)

Figure 1. a) Serial passaging of F81 cells in F81 LSM 801 + 3% NBCS medium at a seeding density of 1.5 × 10⁶ cells/mL, subcultured every 3 days. b) Batch culture of F81 cells in F81 LSM 801 + 3% NBCS medium at a seeding density of 1.5 × 10⁶ cells/mL.

Virus Production

The virus production system developed by JSBio using F81 cells has achieved higher viral titers.

Figure 2. Production of the feline trivalent vaccine viruses—feline herpesvirus (FHV), feline parvovirus (FPV), and feline calicivirus (FCV)—using F81 LSM 801 + 3% NBCS medium, ExpVirus A and ExpVirus B supplements, and suspension-adapted F81 cells.