LMH SFM 777 medium supports the rapid adaptation of LMH adherent cells to suspension culture, enabling stable high-density proliferation and enhancing the intracellular expression efficiency of avian adenovirus. LMH SFM 777 is a serum-free, protein-free, and animal-origin–free medium, significantly reducing downstream purification costs. It has been applied into multiple large-scale production projects of avian adenovirus vaccines.
Figure 1. a) Suspension-adapted LMH cells passaged in LMH SFM 777 medium at a seeding density of 1.0 × 10⁶ cells/mL, subcultured every 3 days. b) LMH suspension cells inoculated at 1.0 × 10⁶ cells/mL in LMH SFM 777 medium reached a peak viable cell density of nearly 9.0 × 10⁶ cells/mL, with cell viability consistently maintained above 95%, which is conducive to subsequent virus inoculation and expression processes.
Figure 2. Avian adenovirus (serotype 4) infection of LMH cells cultured in LMH SFM 777 medium resulted in a maximum viral titer of approximately 10¹⁰ TCID₅₀/mL. Compared with adherent serum-containing cultures (about 10⁸ TCID₅₀/mL), the serum-free suspension system achieved substantially higher viral yields and markedly improved production efficiency.