HEK-293 Cell Culture Media (Recombinant Protein) ​

HEK-293 cells (Human Embryonic Kidney cells) are widely utilized for recombinant protein production and viral vector production (e.g., Adeno-Associated Virus AAV, Adenovirus AdV, Lentivirus LV). To meet their culture requirements, the CD 293 series products (including CD 293 06 medium) are manufactured under GMP conditions. These are chemically defined, animal component-free media that effectively support high-density growth and efficient transfection/expression in various HEK-293 cell lines.

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Product Catalog

Product Catalog No. Form Package Size Description User Guide Inquiry and Trial
CD 293 01 11203-1238、11203-22052 Dry Powder、Liquid 500 mL, 1000 mL, 2 L, 10 L, 50 L, 100 L, Customized Basal Medium Download
CD 293 06 11207-1652、11207-24018 Dry Powder、Liquid 500 mL, 1000 mL, 2 L, 10 L, 50 L, 100 L, Customized Basal Medium Download
CD 293 FA 99151-1541、99151-22055 Dry Powder、Liquid 500 mL, 1000 mL, 2 L, 10 L, 50 L, 100 L, Customized Feed, used with CD 293 FB mainly for stable transfection expression of HEK-293 cells Download
CD 293 FB 99035-1242、99035-23004 Dry Powder、Liquid 500 mL, 1000 mL, 2 L, 10 L, 50 L, 100 L, Customized Feed, used with CD 293 FA or FC, the dosage is 1/10 of CD 293 FA or FC Download
CD 293 FC 99151-1327、99151-23016 Dry Powder、Liquid 500 mL, 1000 mL, 2 L, 10 L, 50 L, 100 L, Customized Feed, used with CD 293 FB mainly for transient transfection expression of HEK-293 cells Download
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Case Studies

HEK-293 Cell Growth

CD 293 01 medium enables robust growth of HEK-293 cells, with a doubling time of approximately 21-24 hours and cell viability exceeding 90%. In batch culture, the maximum cell density can reach 14.0 × 106 cells/mL.

a)

Figure 1.
a) Passage culture of HEK-293 cells using different media, with a seeding density of 0.5 × 106 cells/mL and passage once every 3 days;
b) Batch culture of HEK-293 cells using different media, with a seeding density of 0.5 × 106 cells/mL.

Transfection Efficiency of HEK-293 Cells

Figure 3.
a) FSC-A (cell size)/SSC-A (cell granularity) scatter plot, gating to select P1 cell population;
b) Distribution of fluorescently labeled positive cells in P1 population (positive rate 91.18%)

Protein Expression

CD 293 01 medium significantly enhances transient protein expression in HEK-293 cells. Depending on the molecule and experimental conditions, expression levels range from 50 to 400 mg/L.

Figure 2. Transient expression of various protein molecules by HEK-293 cells using different media. a) Cytokines and growth factors; b) Monoclonal antibodies.