Trans 293 Kit Pro delivered markedly higher expression for all 7 proteins, with several molecules showing more than a twofold increase, underscoring its excellent perfor-mance on the transient transfection of difficult-to-express proteins.
Figure 1. Transient expression of 7 difficult-to-express proteins in HEK293 cells: Trans 293 Kit Pro versus control
Trans 293 Kit Pro consistently achieved higher titers over a 7-day culture period. At Day 7, mAb titer almost reached 2 g/L, representing a 45% increase over the control.
Figure 2. Transient mAb expression in a high-express-ing HEK293 culture: Trans 293 Kit Pro versus control
A comparative transient-expression study of an antibody in HEK293F cells was performed under the following four process conditions:
▪ Single feeding on Day 1
▪ Dual feeding on Day 1 and Day 4
▪ Centrifugation before transfection + single feeding on Day 1
▪Centrifugation before transfection + dual feeding on Day 1 and Day 4
Figure 3. Transient mAb expression across different processes: Trans 293 Kit Pro versus control.