Cells were seeded at 2 × 10⁶ cells/mL one day before transfection and reached 6 × 10⁶ cells/mL on the day of transfection; plasmid concentration was adjusted to 3 µg/mL.
Figure 1. Antibody titer over 7 days in CHO-K1 cells using Trans CHO Kit Plus versus control under a simple process.
Trans CHO Kit Plus delivered significantly higher expres-sion over the 7-day culture, with a 30% improvement in expression level. Titer exceeded 1000 mg/L on Day 5, demonstrating superior transient transfection efficiency and productivity.
Cell density was adjusted to 6 × 10⁶ cells/mL on the day of transfection, and the culture was fed 2–3 times during cultivation.
Cell density was adjusted to 9 × 10⁶ cells/mL on the day of transfection, and the culture was fed 2–3 times during cultivation.
Figure 4. Antibody titer over 11 days in CHO-K1 cells using Trans CHO Kit Plus versus control under a high-density process.
Across the full 11-day culture, Trans CHO Kit Plus maintained significantly higher expression than the control, reaching 4000 mg/L on Day 11 and delivering approximately 30% higher productivity, demonstrating superior transient transfection efficiency and product yield.